Functional Characterization Experiment summary for ENCSR657SBV
doi:10.17989/ENCSR657SBV
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (55-65%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Treatments
- 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-BJAB-True-PPIF-binD_funccharexp
- Date released
- July 11, 2022
- Functional Characterization Series
- ENCSR273LJW
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 2 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS987NMM | ENCGM100KJF, ENCGM324JGL | ENCLB951HHG |
| 2 | 2 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS560ZMS | ENCGM100KJF, ENCGM324JGL | ENCLB166BVF |
| 2 | 1 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS560ZMS | ENCGM100KJF, ENCGM324JGL | ENCLB589REW |
| 2 | 3 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS560ZMS | ENCGM100KJF, ENCGM324JGL | ENCLB015LSM |
| 1 | 1 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS987NMM | ENCGM100KJF, ENCGM324JGL | ENCLB934YCU |
| 2 | 4 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS560ZMS | ENCGM100KJF, ENCGM324JGL | ENCLB042EXB |
| 1 | 3 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS987NMM | ENCGM100KJF, ENCGM324JGL | ENCLB731APS |
| 1 | 4 | Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours | ENCBS987NMM | ENCGM100KJF, ENCGM324JGL | ENCLB939CCS |