Functional Characterization Experiment summary for ENCSR377LWO
doi:10.17989/ENCSR377LWO
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens K562, 7 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA)
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- sparse peak
- Replication type
- isogenic
- Description
- Functional characterization experiment for CRISPRi/dCas9-KRAB-WSR7EEE sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562
- Treatments
- 1 μg/mL Puromycin (CHEBI:17939) for 96 hours
- Nucleic acid type
- DNA
- Extraction method
- QIAGEN DNeasy Blood & Tissue Kit
- Platform
- Illumina NextSeq 550
- Elements references
- Elements cloning
- ENCSR016FPP
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- will-greenleaf:K562_LargeScale_DHS_Growth_dCas9-KRAB-WSR7EEE_T7
- Date released
- December 14, 2021
- Functional Characterization Series
- ENCSR386FFV
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 7 days post-nucleic acid delivery time | ENCBS566HIJ | ENCGM327KLB, ENCGM484HMC | ENCLB958ISI |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 7 days post-nucleic acid delivery time | ENCBS110VZE | ENCGM327KLB, ENCGM484HMC | ENCLB410SWL |