Functional Characterization Experiment summary for ENCSR322MGX

doi:10.17989/ENCSR322MGX

Summary

Status
released
Assay
proliferation CRISPR screen
Biosample summary
Homo sapiens K562, 20 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for CTCF locus
Biosample Type
cell line
Perturbation type
CRISPR cutting
Tiling modality
peak tiling
Replication type
isogenic
Description
Functional characterization experiment for Cas9/CRISPR fine-mapping proliferation screens at hit and non-hit CTCF motifs in loop anchors (and their partner anchors) from the CTCF growth screen
Treatments
1 μg/mL Puromycin (CHEBI:17939) for 96 hours
Nucleic acid type
DNA
Extraction method
QIAGEN DNeasy Blood & Tissue Kit
Elements references
Elements cloning
ENCSR948XFR

Attribution

ENCODE4 project
Lab
Will Greenleaf, Stanford
Award
UM1HG009436 (Will Greenleaf, Stanford)
Project
ENCODE
Aliases
will-greenleaf:K562_CRISPRk_CTCF_FineMap_T20
Date released
December 14, 2021
Functional Characterization Series
ENCSR246NKQ 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 20 days post-nucleic acid delivery timeENCBS488GXVENCGM484HMC, ENCGM924MNTENCLB211POG
21Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 20 days post-nucleic acid delivery timeENCBS405VOVENCGM484HMC, ENCGM924MNTENCLB307ZBI