Functional Characterization Experiment summary for ENCSR298NSD

doi:10.17989/ENCSR298NSD

Summary

Status
released
Assay
Flow-FISH CRISPR screen
Biosample summary
Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
full tiling
Examined loci
HBS1L (0-10%)
CRISPR screen readout
HCR-FlowFISH
Replication type
isogenic
Treatments
1 μg/mL doxycycline (CHEBI:50845) for 48 hours
Nucleic acid type
DNA
Controls
Elements references
  • ENCSR827WZZ (GRCh38 chr6:134253818-136927606, GRCh38 chr11:4091879-6419323)
Elements cloning
ENCSR206MVL

Attribution

ENCODE4 project
Lab
Pardis Sabeti, Broad
Award
UM1HG009435 (Pardis Sabeti, Broad)
Project
ENCODE
Aliases
pardis-sabeti:HBS1L_LS_exp
Date submitted
September 23, 2021
Date released
October 18, 2021
Functional Characterization Series
ENCSR759RSA 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hoursENCBS554UZWENCGM097HOE, ENCGM470LXQENCLB641LHZ
21Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hoursENCBS283LEUENCGM097HOE, ENCGM470LXQENCLB640PVE