Functional Characterization Experiment summary for ENCSR107RAC
doi:10.17989/ENCSR107RAC
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (35-45%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-Jurkat-False-PPIF-binC_funccharexp
- Date released
- July 11, 2022
- Functional Characterization Series
- ENCSR416EUJ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 3 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS071XFR | ENCGM212EML, ENCGM324JGL | ENCLB975BFA |
| 1 | 1 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS071XFR | ENCGM212EML, ENCGM324JGL | ENCLB203RJY |
| 2 | 4 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS509KCR | ENCGM212EML, ENCGM324JGL | ENCLB047ZZB |
| 2 | 2 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS509KCR | ENCGM212EML, ENCGM324JGL | ENCLB542SZL |
| 2 | 3 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS509KCR | ENCGM212EML, ENCGM324JGL | ENCLB912NWW |
| 1 | 2 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS071XFR | ENCGM212EML, ENCGM324JGL | ENCLB522QEB |
| 1 | 4 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS071XFR | ENCGM212EML, ENCGM324JGL | ENCLB007OEZ |
| 2 | 1 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS509KCR | ENCGM212EML, ENCGM324JGL | ENCLB818KZV |