Functional Characterization Experiment summary for ENCSR556AXL
doi:10.17989/ENCSR556AXL
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (55-65%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Treatments
- 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate (CHEBI:37537) for 4 hours
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-Jurkat-True-PPIF-binD_funccharexp
- Date released
- July 11, 2022
- Functional Characterization Series
- ENCSR525DAZ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS225ERP | ENCGM212EML, ENCGM324JGL | ENCLB327HUQ |
| 2 | 3 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS293HNT | ENCGM212EML, ENCGM324JGL | ENCLB804COY |
| 2 | 1 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS293HNT | ENCGM212EML, ENCGM324JGL | ENCLB086BML |
| 2 | 4 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS293HNT | ENCGM212EML, ENCGM324JGL | ENCLB428GVK |
| 1 | 4 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS225ERP | ENCGM212EML, ENCGM324JGL | ENCLB674OQU |
| 2 | 2 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS293HNT | ENCGM212EML, ENCGM324JGL | ENCLB968QRQ |
| 1 | 2 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS225ERP | ENCGM212EML, ENCGM324JGL | ENCLB858ZMZ |
| 1 | 3 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours | ENCBS225ERP | ENCGM212EML, ENCGM324JGL | ENCLB509KHP |