Functional Characterization Experiment summary for ENCSR689KVW
doi:10.17989/ENCSR689KVW
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens OCI-AML2 genetically modified (insertion) using transduction, using CRISPRi (sgRNA)
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- sparse peak
- Replication type
- isogenic
- Description
- CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in OCI-AML2 acute myeloid leukemia cells
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- References
- Aliases
- charles-gersbach:encode4-ceres-dcas9-krab-validation-screen-ociaml2
- Date submitted
- August 31, 2021
- Date released
- October 20, 2022
- Functional Characterization Series
- ENCSR179FSH
- Submitter comment
- dCas9-KRAB
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS091HPB | ENCGM710IEC, ENCGM809XEK | ENCLB639QZP |
| 3 | 1 | Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS720FRS | ENCGM710IEC, ENCGM809XEK | ENCLB517IHN |
| 2 | 1 | Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS530LLD | ENCGM710IEC, ENCGM809XEK | ENCLB190BOQ |
| 4 | 1 | Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS554DTM | ENCGM710IEC, ENCGM809XEK | ENCLB581WFS |