Functional Characterization Experiment summary for ENCSR770ENU

doi:10.17989/ENCSR770ENU

Summary

Status
released
Assay
CRISPR screen (Control CRISPR screen)
Control type
control
Biosample summary
Homo sapiens OCI-AML2 genetically modified (insertion) using transduction
Biosample Type
cell line
Tiling modality
sparse peak
Replication type
isogenic
Description
CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in OCI-AML2 acute myeloid leukemia cells
Nucleic acid type
DNA
Elements references

Attribution

ENCODE4 project
Lab
Tim Reddy, Duke
Award
UM1HG009428 (Tim Reddy, Duke)
Project
ENCODE
Aliases
charles-gersbach:encode4-ceres-wt-validation-screen-ociaml2
Date submitted
August 31, 2021
Date released
October 20, 2022
Functional Characterization Series
ENCSR179FSH 
Submitter comment
Wild type

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transductionENCBS460CKDENCGM809XEKENCLB637NTI
31Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transductionENCBS865EGSENCGM809XEKENCLB212YDG
41Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transductionENCBS026SNIENCGM809XEKENCLB390TKS
11Homo sapiens OCI-AML2 cell line genetically modified (insertion) using transductionENCBS680UAAENCGM809XEKENCLB855ANU