Functional Characterization Experiment summary for ENCSR397CXJ
doi:10.17989/ENCSR397CXJ
Supersedes ENCSR833EYM
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRa (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRa
- Tiling modality
- full tiling
- Replication type
- isogenic
- Description
- Tiling, dCas9-VPR/CRISPRa proliferation screen at the GATA1, MYB, and ZMYND8 loci
- Treatments
- 1 μg/mL Puromycin (CHEBI:17939) for 96 hours
- Nucleic acid type
- DNA
- Extraction method
- QIAGEN DNeasy Blood & Tissue Kit
- Platform
- Illumina HiSeq 4000
- Elements references
- ENCSR145WNY (GATA1, MYB, ZMYND8)
- Elements cloning
- ENCSR479VDK
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- References
- Aliases
- will-greenleaf:CRISPR_GATA1_tiling.CRISPRa_T21
- Date released
- December 14, 2021
- Functional Characterization Series
- ENCSR882EWB
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRa (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery time | ENCBS074QCT | ENCGM440XJM, ENCGM484HMC | ENCLB025SBM |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRa (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery time | ENCBS409YJF | ENCGM440XJM, ENCGM484HMC | ENCLB543BBG |