Functional Characterization Experiment summary for ENCSR904DYJ

doi:10.17989/ENCSR904DYJ

Summary

Status
released
Assay
proliferation CRISPR screen
Biosample summary
Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for CTCF locus
Biosample Type
cell line
Perturbation type
CRISPR cutting
Tiling modality
sparse peak
Replication type
isogenic
Description
Functional characterization experiment for tiling, Cas9/CRISPRk proliferation screens at CTCF motifs in loop anchors
Treatments
1 μg/mL Puromycin (CHEBI:17939) for 96 hours
Nucleic acid type
DNA
Extraction method
QIAGEN DNeasy Blood & Tissue Kit
Elements references
Elements cloning
ENCSR067VXW

Attribution

ENCODE4 project
Lab
Will Greenleaf, Stanford
Award
UM1HG009436 (Will Greenleaf, Stanford)
Project
ENCODE
Aliases
will-greenleaf:K562_CRISPRk_CTCF_Growth_T21
Date released
December 14, 2021
Functional Characterization Series
ENCSR022LUE 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery timeENCBS731THVENCGM484HMC, ENCGM924MNTENCLB197IKH
21Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery timeENCBS101ONFENCGM484HMC, ENCGM924MNTENCLB286FPF