Functional Characterization Experiment summary for ENCSR664UQJ
doi:10.17989/ENCSR664UQJ
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (90-100%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Treatments
- 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-THP1-True-PPIF-binF_funccharexp
- Date released
- May 10, 2022
- Functional Characterization Series
- ENCSR108MPF
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
1 | 2 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS607AOV | ENCGM879NCV, ENCGM324JGL | ENCLB312YOL |
2 | 2 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS414KPG | ENCGM879NCV, ENCGM324JGL | ENCLB279BZJ |
2 | 3 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS414KPG | ENCGM879NCV, ENCGM324JGL | ENCLB970DWF |
1 | 4 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS607AOV | ENCGM879NCV, ENCGM324JGL | ENCLB974LCP |
2 | 1 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS414KPG | ENCGM879NCV, ENCGM324JGL | ENCLB212HGB |
1 | 1 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS607AOV | ENCGM879NCV, ENCGM324JGL | ENCLB247XIO |
1 | 3 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS607AOV | ENCGM879NCV, ENCGM324JGL | ENCLB054WLP |
2 | 4 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS414KPG | ENCGM879NCV, ENCGM324JGL | ENCLB481RVL |