Functional Characterization Experiment summary for ENCSR509FZL

doi:10.17989/ENCSR509FZL

Summary

Status
released
Assay
proliferation CRISPR screen
Biosample summary
Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA)
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
sparse peak
Replication type
isogenic
Description
Functional characterization experiment for CRISPRi/dCas9-KRAB-MGA1-MGA2 sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562
Treatments
1 μg/mL Puromycin (CHEBI:17939) for 96 hours
Nucleic acid type
DNA
Extraction method
QIAGEN DNeasy Blood & Tissue Kit
Elements references
Elements cloning
ENCSR729ITW

Attribution

ENCODE4 project
Lab
Will Greenleaf, Stanford
Award
UM1HG009436 (Will Greenleaf, Stanford)
Project
ENCODE
Aliases
will-greenleaf:K562_LargeScale_DHS_Growth_dCas9-KRAB-MGA1-MGA2_T21
Date released
December 14, 2021
Functional Characterization Series
ENCSR427OCU 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery timeENCBS585LHKENCGM484HMC, ENCGM149QCDENCLB558QFM
21Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery timeENCBS333TCOENCGM484HMC, ENCGM149QCDENCLB289USB