Functional Characterization Experiment summary for ENCSR162HMH
doi:10.17989/ENCSR162HMH
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA)
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Replication type
- isogenic
- Description
- CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen all >100,000 putative non-coding regulatory elements defined by open chromatin sites in human K562 leukemia cells for their role in regulating essential cellular processes (whole genome).
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- charles-gersbach:encode4-ceres-dcas9-krab-wg-screen-k562
- Date submitted
- August 31, 2021
- Date released
- October 20, 2022
- Functional Characterization Series
- ENCSR690DTG
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 4 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS314PQK | ENCGM710IEC, ENCGM809XEK | ENCLB382MQL |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS281SVF | ENCGM710IEC, ENCGM809XEK | ENCLB138CPP |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS047JRA | ENCGM710IEC, ENCGM809XEK | ENCLB235GYC |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS370SPY | ENCGM710IEC, ENCGM809XEK | ENCLB387PCC |