Functional Characterization Experiment summary for ENCSR245TFD
doi:10.17989/ENCSR245TFD
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (0-10%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-Jurkat-False-PPIF-binA_funccharexp
- Date released
- July 11, 2022
- Functional Characterization Series
- ENCSR416EUJ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 3 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS146BOI | ENCGM212EML, ENCGM324JGL | ENCLB545GMT |
| 1 | 2 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS146BOI | ENCGM212EML, ENCGM324JGL | ENCLB457OHR |
| 2 | 3 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS317YLV | ENCGM212EML, ENCGM324JGL | ENCLB280PBF |
| 2 | 4 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS317YLV | ENCGM212EML, ENCGM324JGL | ENCLB095FUL |
| 1 | 4 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS146BOI | ENCGM212EML, ENCGM324JGL | ENCLB215CYO |
| 2 | 2 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS317YLV | ENCGM212EML, ENCGM324JGL | ENCLB864ZWP |
| 1 | 1 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS146BOI | ENCGM212EML, ENCGM324JGL | ENCLB006GMP |
| 2 | 1 | Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS317YLV | ENCGM212EML, ENCGM324JGL | ENCLB825IXJ |