1. Functional Characterization Experiments
  2. Flow-FISH CRISPR screen
  3. Homo sapiens
  4. THP-1

Functional Characterization Experiment summary for ENCSR473PCZ

doi:10.17989/ENCSR473PCZ

Summary

Status
released
Assay
Flow-FISH CRISPR screen
Biosample summary
Homo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
peak tiling
Examined loci
PPIF (35-45%)
CRISPR screen readout
PrimeFlow
Replication type
isogenic
Treatments
1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours
Nucleic acid type
DNA
Size range
200-210
Elements references

Attribution

ENCODE4 project
Lab
Jesse Engreitz, Stanford
Award
UM1HG009436 (Will Greenleaf, Stanford)
Project
ENCODE
Aliases
jesse-engreitz:CRISPRiFlowFISH-THP1-True-PPIF-binC_funccharexp
Date released
May 10, 2022
Functional Characterization Series
ENCSR108MPF 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
14Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS101MFPENCGM879NCV, ENCGM324JGLENCLB689UWL
24Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS250KFFENCGM879NCV, ENCGM324JGLENCLB578OOV
13Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS101MFPENCGM879NCV, ENCGM324JGLENCLB781KTF
21Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS250KFFENCGM879NCV, ENCGM324JGLENCLB932SIL
11Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS101MFPENCGM879NCV, ENCGM324JGLENCLB872NHA
22Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS250KFFENCGM879NCV, ENCGM324JGLENCLB849NGG
12Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS101MFPENCGM879NCV, ENCGM324JGLENCLB505FZC
23Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hoursENCBS250KFFENCGM879NCV, ENCGM324JGLENCLB103ZVS

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