ENCBS101MFP / cell line
Summary
- Status
- released
- Term name
- THP-1
- Term ID
- EFO:0001253
- Summary
- Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award PI
- Will Greenleaf, Stanford
- Submitted by
- Benjamin Doughty
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-THP1-True-PPIF-biorep1-binC_biosamp
Treatment details
- Treatment
- 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours
- Type
- chemical
- Purpose
- stimulation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM879NCV | CRISPRi | characterization | CRISPR | ||
| ENCGM324JGL | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO303OIJ
- Aliases
- michael-snyder:thp-1-donor
- Species
- Homo sapiens
- Life stage
- child
- Age
- 1 year
- Sex
- male
- Health status
- acute monocytic leukemia
- External resources