Functional Characterization Experiment summary for ENCSR294JZL
doi:10.17989/ENCSR294JZL
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPR dCas (sgRNA)
- Biosample Type
- cell line
- Perturbation type
- CRISPR dCas
- Tiling modality
- peak tiling
- Replication type
- isogenic
- Description
- Functional characterization experiment for dCas9 tiling proliferation screens at a subset of cCREs around essential genes
- Treatments
- 1 μg/mL Puromycin (CHEBI:17939) for 96 hours
- Nucleic acid type
- DNA
- Extraction method
- QIAGEN DNeasy Blood & Tissue Kit
- Platform
- Illumina NextSeq 550
- Elements references
- Elements cloning
- ENCSR024GFN
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- will-greenleaf:K562_DHS_BatchTiling_Growth_dCas9_T21
- Date released
- December 14, 2021
- Functional Characterization Series
- ENCSR997ZOY
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR dCas (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery time | ENCBS991RDE | ENCGM484HMC, ENCGM641LWQ | ENCLB848RMW |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR dCas (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery time | ENCBS190BYF | ENCGM484HMC, ENCGM641LWQ | ENCLB187JFQ |