Functional Characterization Experiment summary for ENCSR171CNP
doi:10.17989/ENCSR171CNP
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (80-90%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Treatments
- 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-THP1-True-PPIF-binE_funccharexp
- Date released
- May 10, 2022
- Functional Characterization Series
- ENCSR108MPF
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 2 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS262UKC | ENCGM879NCV, ENCGM324JGL | ENCLB950XBM |
| 2 | 4 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS576TZS | ENCGM879NCV, ENCGM324JGL | ENCLB225TPW |
| 1 | 3 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS262UKC | ENCGM879NCV, ENCGM324JGL | ENCLB239FEQ |
| 2 | 2 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS576TZS | ENCGM879NCV, ENCGM324JGL | ENCLB971RET |
| 1 | 4 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS262UKC | ENCGM879NCV, ENCGM324JGL | ENCLB198MHN |
| 2 | 1 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS576TZS | ENCGM879NCV, ENCGM324JGL | ENCLB938QWA |
| 2 | 3 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS576TZS | ENCGM879NCV, ENCGM324JGL | ENCLB397WZR |
| 1 | 1 | Homo sapiens THP-1 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Lipopolysaccharide from Escherichia coli K12 for 4 hours | ENCBS262UKC | ENCGM879NCV, ENCGM324JGL | ENCLB915BMQ |