Functional Characterization Experiment summary for ENCSR745RFL
doi:10.17989/ENCSR745RFL
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locus
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PPIF (35-45%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
- Elements references
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-GM12878-False-PPIF-binC_funccharexp
- Date released
- May 7, 2022
- Functional Characterization Series
- ENCSR194SMN
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 3 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS532CHT | ENCGM772UBR, ENCGM324JGL | ENCLB152BFE |
| 2 | 1 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS846PNK | ENCGM772UBR, ENCGM324JGL | ENCLB452JGK |
| 2 | 4 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS846PNK | ENCGM772UBR, ENCGM324JGL | ENCLB764MTK |
| 2 | 3 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS846PNK | ENCGM772UBR, ENCGM324JGL | ENCLB729IEE |
| 1 | 2 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS532CHT | ENCGM772UBR, ENCGM324JGL | ENCLB503QGW |
| 1 | 4 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS532CHT | ENCGM772UBR, ENCGM324JGL | ENCLB037XAI |
| 1 | 1 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS532CHT | ENCGM772UBR, ENCGM324JGL | ENCLB711CVZ |
| 2 | 2 | Homo sapiens GM12878 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS846PNK | ENCGM772UBR, ENCGM324JGL | ENCLB739NBI |