Functional Characterization Experiment summary for ENCSR346PHE
doi:10.17989/ENCSR346PHE
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- full tiling
- Examined loci
- HBE1 (90-100%)
- CRISPR screen readout
- HCR-FlowFISH
- Replication type
- isogenic
- Treatments
- 1 μg/mL doxycycline (CHEBI:50845) for 48 hours
- Nucleic acid type
- DNA
- Platform
- Illumina NextSeq 500, Illumina MiSeq
- Controls
- Elements references
- ENCSR827WZZ (GRCh38 chr6:134253818-136927606, GRCh38 chr11:4091879-6419323)
- Elements cloning
- ENCSR206MVL
Attribution
ENCODE4 project
- Lab
- Pardis Sabeti, Broad
- Award
- UM1HG009435 (Pardis Sabeti, Broad)
- Project
- ENCODE
- Aliases
- pardis-sabeti:HBE1_HS_exp
- Date submitted
- September 23, 2021
- Date released
- October 18, 2021
- Functional Characterization Series
- ENCSR564EPW
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hours | ENCBS815UTF | ENCGM097HOE, ENCGM470LXQ | ENCLB768UQH |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hours | ENCBS059EJR | ENCGM097HOE, ENCGM470LXQ | ENCLB157KQT |