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- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 7 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: synthetic elementsLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of ZFAND3 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZFAND3 enhancer.
- MPRA in Neuro-2aElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ultra conserved element UC88.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of TERT locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TCF7L2 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TCF7L2 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT enhancer, using 3' to 5' direction.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of RET locus in Neuro-2aElements cloned into the pGL3 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the RET enhancer.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of MYC locus in HEK293TElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs6983267.
- MPRA of MYC locus in LNCAPElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs11986220.
- MPRA of MSMB locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MSMB promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of IRF6 locus in HaCaTElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF6 enhancer.
- MPRA of IRF4 locus in SK-MEL-28Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF4 enhancer.
- MPRA of HNF4A locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
- MPRA of HBG1 locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBG1 promoter.
- MPRA of HBB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBB promoter.
- MPRA of GP1BB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the GP1BB promoter.
- MPRA of FOXE1 locus in HeLaElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the FOXE1 promoter.
- MPRA of F9 locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the F9 promoter.
- MPRA of BCL11A locus in HELElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the BCL11A enhancer.
- CRISPR cutting CRISPR screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting CRISPR screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting CRISPR screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- CRISPR cutting CRISPR screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYBHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYBHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBS1LHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBS1LHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBE1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBE1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG2Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG2Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-RYBP sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-KRAB-WSR7EEE sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-RYBP sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-KRAB-WSR7EEE sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-KRAB-MGA1-MGA2 sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-ZNF705-KRAB/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-RYBP/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-KRAB-WSR7EEE/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-KRAB/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR dCas proliferation screen in K562Functional characterization experiment for dCas9 tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for Cas9/CRISPR fine-mapping proliferation screens at hit and non-hit CTCF motifs in loop anchors (and their partner anchors) from the CTCF growth screenElements selection method: TF binding sitesLoci: CTCFTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for Cas9/CRISPR fine-mapping proliferation screens at hit and non-hit CTCF motifs in loop anchors (and their partner anchors) from the CTCF growth screenElements selection method: TF binding sitesLoci: CTCFTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, Cas9/CRISPRk proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, Cas9/CRISPRk proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-KRAB/CRISPRi proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-KRAB/CRISPRi proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR dCas proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9/CRISPRd proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR dCas proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9/CRISPRd proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRa proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-VPR/CRISPRa proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRa proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-VPR/CRISPRa proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 14 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2 originated from WTC11Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in WTC11Homo sapiens WTC11 genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- CRISPR dCas proliferation screen of multiple loci in K562Tiling, dCas9/CRISPRd proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPR dCas proliferation screen of multiple loci in K562Tiling, dCas9/CRISPRd proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRa proliferation screen of multiple loci in K562Tiling, dCas9-VPR/CRISPRa proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRa proliferation screen of multiple loci in K562Tiling, dCas9-VPR/CRISPRa proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPR cutting proliferation screen of multiple loci in K562Tiling, Cas9/CRISPRk proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPR cutting proliferation screen of multiple loci in K562Tiling, Cas9/CRISPRk proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRi proliferation screen of multiple loci in K562Tiling, dCas9-KRAB/CRISPRi proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRi proliferation screen of multiple loci in K562Tiling, dCas9-KRAB/CRISPRi proliferation screen at the GATA1, MYB, and ZMYND8 loci
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- CRISPR cutting proliferation screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting proliferation screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- CRISPR cutting proliferation screen of MYC locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting FACS screen of MYC locus in K562 with endogenous protein Sort-seq readout of MYCCRISPR screen targeting candidate CREs following FACS high
- CRISPR cutting FACS screen of MYC locus in K562 with endogenous protein Sort-seq readout of MYCCRISPR screen targeting candidate CREs following FACS low
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA of multiple loci in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection for multiple loci
- MPRA in HCT116Homo sapiens HCT116, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HCT116Homo sapiens HCT116, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- perturbation followed by snATAC-seq in GM12878CRISPRi perturbation of GM12878sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in MCF-7CRISPRi perturbation of MCF7sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by scRNA-seq in K562Homo sapiens K562 genetically modified (CRISPRi) using CRISPRLab: Jay Shendure, UWProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM ZK216348 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM ZK216348 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM RU486 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Mapracorat (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM Mapracorat for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM Hydrocortisone for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM GW870086 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM GW870086 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM dexamethasone (CHEBI:41879) for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM CpdA (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM CpdA for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM CORT108297 (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM CORT108297 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM AZD9567 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM AZD9567 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM AZD2906 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM AZD2906 for 4 hours
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi proliferation screen in OCI-AML2CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in OCI-AML2 acute myeloid leukemia cellsTiling modality: sparse peakLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi proliferation screen in K562CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in K562 leukemia cellsTiling modality: sparse peakLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi proliferation screen in K562CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen all >100,000 putative non-coding regulatory elements defined by open chromatin sites in human K562 leukemia cells for their role in regulating essential cellular processes (whole genome).Tiling modality: peak tilingLab: Tim Reddy, DukeProject: ENCODE
- perturbation followed by scRNA-seq in K562Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA)Lab: Tim Reddy, DukeProject: ENCODELibrary construction platform: 10X Genomics Chromium Controller
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CAPRIN1CAPRIN1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CAPRIN1CAPRIN1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CATCATTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CATCATTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of PVT1PVT1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYCMYCTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE