Showing 98 of 98 results
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- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 7 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of ZFAND3 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZFAND3 enhancer.
- MPRA in Neuro-2aElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ultra conserved element UC88.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TCF7L2 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TCF7L2 enhancer.
- MPRA of RET locus in Neuro-2aElements cloned into the pGL3 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the RET enhancer.
- MPRA of MYC locus in HEK293TElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs6983267.
- MPRA of MSMB locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MSMB promoter.
- MPRA of IRF6 locus in HaCaTElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF6 enhancer.
- MPRA of HNF4A locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 14 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2 originated from WTC11Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in WTC11Homo sapiens WTC11 genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in activated CD4-positive, CD25-positive, alpha-beta regulatory T cellMus musculus strain C57BL/6NJ activated CD4-positive, CD25-positive, alpha-beta regulatory T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 2 cellMus musculus strain C57BL/6NJ activated T-helper 2 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 1 cellMus musculus strain C57BL/6NJ activated T-helper 1 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated naive CD4-positive, alpha-beta T cellMus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in mammary epithelial cellHomo sapiens mammary epithelial cell female genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- perturbation followed by snATAC-seq in GM12878CRISPRi perturbation of GM12878sLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting FACS screen of Sox2 locus in F123 with endogenous protein Sort-seq readout of Sox2Mus musculus F123 genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Sox2, (insertion) using transduction, using CRISPR cutting (sgRNA) for Sox2 locusElements selection method: DNase hypersensitive sitesLoci: Sox2Tiling modality: peak tilingLab: Yin Shen, UCSFProject: ENCODE
- CRISPR cutting FACS screen of Esrrb locus in F123 with endogenous protein Sort-seq readout of EsrrbMus musculus F123 genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Esrrb, (insertion) using transduction, using CRISPR cutting (sgRNA) for Esrrb locusElements selection method: DNase hypersensitive sitesLoci: EsrrbTiling modality: peak tilingLab: Yin Shen, UCSFProject: ENCODE
- CRISPR cutting FACS screen of Sox2 locus in F123 with endogenous protein Sort-seq readout of Sox2Mus musculus F123 genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Sox2, (insertion) using transduction, using CRISPR cutting (sgRNA) for Sox2 locusElements selection method: DNase hypersensitive sitesLoci: Sox2Tiling modality: peak tilingLab: Yin Shen, UCSFProject: ENCODE
- CRISPR cutting FACS screen of Esrrb locus in F123 with endogenous protein Sort-seq readout of EsrrbMus musculus F123 genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Esrrb, (insertion) using transduction, using CRISPR cutting (sgRNA) for Esrrb locusElements selection method: DNase hypersensitive sitesLoci: EsrrbTiling modality: peak tilingLab: Yin Shen, UCSFProject: ENCODE
- CRISPR cutting FACS screen of Dppa2 locus in F123 with endogenous protein Sort-seq readout of Dppa2Mus musculus F123 genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Dppa2, (insertion) using transduction, using CRISPR cutting (sgRNA) for Dppa2 locusElements selection method: DNase hypersensitive sitesLoci: Dppa2Tiling modality: peak tilingLab: Yin Shen, UCSFProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in JurkatHomo sapiens Jurkat, 48 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- CRISPR cutting FACS screen of FMR1 locus in glutamatergic neuron with endogenous protein Sort-seq readout of FMR1Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens FMR1, (insertion) using transduction, using CRISPR cutting (pgRNA) for FMR1 locus
- CRISPR cutting FACS screen of APP locus in glutamatergic neuron with endogenous protein Sort-seq readout of APPHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in glutamatergic neuron with endogenous protein Sort-seq readout of SIN3AHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of MECP2 locus in glutamatergic neuron with endogenous protein Sort-seq readout of MECP2Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- CRISPR cutting FACS screen of APP locus in glutamatergic neuron with endogenous protein Sort-seq readout of APPHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in glutamatergic neuron with endogenous protein Sort-seq readout of SIN3AHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of FMR1 locus in WTC11 with endogenous protein Sort-seq readout of FMR1Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens FMR1, (insertion) using transduction, using CRISPR cutting (pgRNA) for FMR1 locus
- CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APPHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3AHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of MECP2 locus in WTC11 with endogenous protein Sort-seq readout of MECP2Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APPHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3AHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- STARR-seq in SH-SY5YHomo sapiens SH-SY5Y genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE