Functional Characterization Experiment summary for ENCSR063AQH

doi:10.17989/ENCSR063AQH

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Mus musculus F123 genetically modified (disruption) using CRISPR (sgRNA), (insertion) using CRISPR (sgRNA) targeting M. musculus Esrrb, (insertion) using transduction for Esrrb locus
Biosample Type
cell line
Perturbation type
disruption
Examined loci
Esrrb (1st percentile)
CRISPR screen readout
endogenous protein Sort-seq
Replication type
isogenic
Nucleic acid type
DNA
Controls
Elements references
Elements cloning
ENCSR215PWC

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
yin-shen:Low eGFP sorted cells assay_Esrrb
Date submitted
July 30, 2020
Date released
August 7, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Mus musculus F123 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Esrrb, genetically modified (insertion) using transductionENCBS915RDIENCGM132ERD, ENCGM747RQP, ENCGM205ZAOENCLB750TAZ
21Mus musculus F123 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Esrrb, genetically modified (insertion) using transductionENCBS736ICLENCGM132ERD, ENCGM747RQP, ENCGM205ZAOENCLB362UNP