Functional Characterization Experiment summary for ENCSR897BBN

doi:10.17989/ENCSR897BBN

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Mus musculus F123 genetically modified (disruption) using CRISPR (sgRNA), (insertion) using CRISPR (sgRNA) targeting M. musculus Sox2, (insertion) using transduction for Sox2 locus
Biosample Type
cell line
Perturbation type
disruption
Examined loci
Sox2 (1st percentile)
CRISPR screen readout
endogenous protein Sort-seq
Replication type
isogenic
Nucleic acid type
DNA
Controls
Elements references
Elements cloning
ENCSR919NIR

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
yin-shen:Low mCherry sorted assay_Sox2
Date submitted
July 30, 2020
Date released
August 7, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Mus musculus F123 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Sox2, genetically modified (insertion) using transductionENCBS026NQQENCGM710YTX, ENCGM168QBG, ENCGM221OORENCLB438ZDU
21Mus musculus F123 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Sox2, genetically modified (insertion) using transductionENCBS707MVYENCGM710YTX, ENCGM168QBG, ENCGM221OORENCLB857UTQ