Functional Characterization Experiment summary for ENCSR552KGE

doi:10.17989/ENCSR552KGE

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Mus musculus Castaneus X S129/SvJae F123 genetically modified using CRISPR (sgRNA), transduction
Biosample Type
cell line
Perturbation type
disruption
Examined loci
Dppa2 (1st percentile)
Replication type
isogenic
Nucleic acid type
DNA
Controls
Elements references
Elements cloning
ENCSR147EBW

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
yin-shen:Low mCherry sorted assay_Dppa2
Date submitted
July 30, 2020
Date released
August 7, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11F123 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using CRISPR (sgRNA) targeting Dppa2, genetically modified (insertion) using transductionENCBS572NGFENCGM364WLO, ENCGM802UGA, ENCGM896QXRENCLB821KVZ
21F123 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using CRISPR (sgRNA) targeting Dppa2, genetically modified (insertion) using transductionENCBS411FGLENCGM364WLO, ENCGM802UGA, ENCGM896QXRENCLB133WLH