Showing 100 of 106 results
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- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: synthetic elementsLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of ZFAND3 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZFAND3 enhancer.
- MPRA in Neuro-2aElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ultra conserved element UC88.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TCF7L2 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TCF7L2 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT enhancer, using 3' to 5' direction.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of RET locus in Neuro-2aElements cloned into the pGL3 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the RET enhancer.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of MYC locus in HEK293TElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs6983267.
- MPRA of MYC locus in LNCAPElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs11986220.
- MPRA of MSMB locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MSMB promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of IRF6 locus in HaCaTElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF6 enhancer.
- MPRA of IRF4 locus in SK-MEL-28Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF4 enhancer.
- MPRA of HNF4A locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
- MPRA of HBG1 locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBG1 promoter.
- MPRA of HBB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBB promoter.
- MPRA of GP1BB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the GP1BB promoter.
- MPRA of FOXE1 locus in HeLaElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the FOXE1 promoter.
- MPRA of F9 locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the F9 promoter.
- MPRA of BCL11A locus in HELElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the BCL11A enhancer.
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYBHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYBHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBS1LHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBS1LHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBE1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBE1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG2Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG2Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated CD4-positive, CD25-positive, alpha-beta regulatory T cellMus musculus strain C57BL/6NJ activated CD4-positive, CD25-positive, alpha-beta regulatory T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 2 cellMus musculus strain C57BL/6NJ activated T-helper 2 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 1 cellMus musculus strain C57BL/6NJ activated T-helper 1 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated naive CD4-positive, alpha-beta T cellMus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CAPRIN1CAPRIN1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CAPRIN1CAPRIN1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CATCATTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CATCATTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of PVT1PVT1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYCMYCTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of PVT1PVT1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYCMYCTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of a mix of short, medium, and long elements in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of the same set of elements in both the forward and reverse orientations in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements and barcodes cloned into the 3' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements and barcodes cloned into the 3' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 3' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 3' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 5' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 5' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. Tested in the same batch as ORI_b2, using primers around the full element and barcode to circumvent the possible detection of artifacts emerging from transcription initiation internal to the upstream element.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as ORI, and serves as an internal control to ORI_full.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in the same batch as HSS_b2, using primers around the full element and barcode to circumvent the possible detection of artifacts emerging from transcription initiation internal to the upstream element.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as HSS, and serves as an internal control to HSS_full.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 48 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in SK-N-SHHomo sapiens SK-N-SH, 48 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in JurkatHomo sapiens Jurkat, 48 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 48 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA of multiple loci in K562Homo sapiens K562, 48 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection for multiple loci
- STARR-seq in K562Human gDNA fragments were selected based on DHS data, GRO-cap data, and/or histone marks in K562 cells and tested for their enhancer activity with eSTARR-seq. The RNAs derived from the transfected plasmids were sequenced and reported here.Elements selection method: DNase hypersensitive sites, transcription start sites, chromatin statesLab: Haiyuan Yu, CornellProject: ENCODE
- STARR-seq in K562Human gDNA fragments were selected based on DHS data, GRO-cap data, and/or histone marks in K562 cells and tested for their enhancer activity with eSTARR-seq. The RNAs derived from the transfected plasmids were sequenced and reported here.Elements selection method: DNase hypersensitive sites, transcription start sites, chromatin statesLab: Haiyuan Yu, CornellProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of NMUNMUTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of NMUNMUTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MEF2CMEF2CTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MEF2CMEF2CTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of LMO2LMO2Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of LMO2LMO2Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of ERP29ERP29Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE