Functional Characterization Experiment summary for ENCSR640NMZ
doi:10.17989/ENCSR640NMZ
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for chr4:55237572-56068533
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- full tiling
- Examined loci
- NMU (90-100%)
- CRISPR screen readout
- HCR-FlowFISH
- Replication type
- isogenic
- Description
- NMU
- Treatments
- 1 μg/mL doxycycline (CHEBI:50845) for 48 hours
- Nucleic acid type
- DNA
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- ENCSR733BIG (GRCh38 chr4:55237572-56068533)
- Elements cloning
- ENCSR631QFN
Attribution
ENCODE4 project
- Lab
- Pardis Sabeti, Broad
- Award
- UM1HG009435 (Pardis Sabeti, Broad)
- Project
- ENCODE
- Aliases
- pardis-sabeti:NMU_HS_exp
- Date submitted
- May 9, 2020
- Date released
- May 20, 2021
- Functional Characterization Series
- ENCSR393VDD
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hours | ENCBS187ALX | ENCGM097HOE, ENCGM470LXQ | ENCLB800EJB |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hours | ENCBS347CID | ENCGM097HOE, ENCGM470LXQ | ENCLB087SEE |