Functional Characterization Experiment summary for ENCSR425RUY

doi:10.17989/ENCSR425RUY

Summary

Status
released
Assay
Flow-FISH CRISPR screen
Biosample summary
Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for chr8:126736096-128735246
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
full tiling
Examined loci
MYC (0-10%)
CRISPR screen readout
HCR-FlowFISH
Replication type
isogenic
Description
MYC
Treatments
1 μg/mL doxycycline (CHEBI:50845) for 48 hours
Nucleic acid type
DNA
Controls
Elements references
Elements cloning
ENCSR782RDD

Attribution

ENCODE4 project
Lab
Pardis Sabeti, Broad
Award
UM1HG009435 (Pardis Sabeti, Broad)
Project
ENCODE
Aliases
pardis-sabeti:MYC_LS_exp
Date submitted
December 4, 2020
Date released
May 20, 2021
Functional Characterization Series
ENCSR825SFH 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hoursENCBS161HTNENCGM097HOE, ENCGM470LXQENCLB798OCC
21Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hoursENCBS449DDDENCGM097HOE, ENCGM470LXQENCLB901MDE