Functional Characterization Experiment summary for ENCSR514FNW

doi:10.17989/ENCSR514FNW

Summary

Status
released
Assay
STARR-seq
Biosample summary
Homo sapiens K562 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Human gDNA fragments were selected based on DHS data, GRO-cap data, and/or histone marks in K562 cells and tested for their enhancer activity with eSTARR-seq. The RNAs derived from the transfected plasmids were sequenced and reported here.
Nucleic acid type
RNA
Size range
150-600
Fragmentation methods
chemical (Tn5 transposase)
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Haiyuan Yu, Cornell
Award
UM1HG009393 (John Lis, Cornell)
Project
ENCODE
Aliases
john-lis:eSTARR1_MYC_RNA
Date submitted
July 8, 2020
Date released
July 13, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21K562 cell line genetically modified (episome) using transient transfectionENCBS616VDWENCGM349ZNDENCLB900EMO
11K562 cell line genetically modified (episome) using transient transfectionENCBS011YRZENCGM349ZNDENCLB145ROZ
31K562 cell line genetically modified (episome) using transient transfectionENCBS661YGRENCGM349ZNDENCLB316STV