Functional Characterization Experiment summary for ENCSR306NNT
doi:10.17989/ENCSR306NNT
Summary
- Status
- released
- Assay
- STARR-seq (Control STARR-seq)
- Control type
- control
- Biosample summary
- Homo sapiens K562 genetically modified (episome) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Human gDNA fragments were selected based on DHS data, GRO-cap data, and/or histone marks in K562 cells and tested for their enhancer activity with eSTARR-seq. The transfected plasmids (post-transfection DNA sample) were sequenced and reported here.
- Nucleic acid type
- DNA
- Size range
- 150-600
- Fragmentation methods
- chemical (Tn5 transposase)
- Platform
- Illumina NextSeq 500
- Elements references
Attribution
ENCODE4 project
- Lab
- Haiyuan Yu, Cornell
- Award
- UM1HG009393 (John Lis, Cornell)
- Project
- ENCODE
- Aliases
- john-lis:eSTARR1_MYC_DNA
- Date submitted
- July 8, 2020
- Date released
- July 13, 2020
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
2 | 1 | Homo sapiens K562 cell line genetically modified (episome) using transient transfection | ENCBS616VDW | ENCGM349ZND | ENCLB613JKA |
3 | 1 | Homo sapiens K562 cell line genetically modified (episome) using transient transfection | ENCBS661YGR | ENCGM349ZND | ENCLB188MQB |
1 | 1 | Homo sapiens K562 cell line genetically modified (episome) using transient transfection | ENCBS011YRZ | ENCGM349ZND | ENCLB653AUJ |