Experiment summary for ENCSR592KSP

Summary

Status
revoked
Assay
ChIP-seq (TF ChIP-seq)
Target
ZFP64
Biosample summary
Homo sapiens HEK293 genetically modified (insertion) using site-specific recombination targeting H. sapiens ZFP64
Biosample Type
cell line
Replication type
isogenic
Description
eGFP-ZFP64 ChIP-seq on human HEK293 cells.
Nucleic acid type
DNA
Size range
450-650

Attribution

ENCODE3 project
Lab
Michael Snyder, Stanford
Award
U54HG006996 (Michael Snyder, Stanford)
Project
ENCODE
External resources
Aliases
michael-snyder:HEK_eGFP-ZFP64_FRT_ChIP
Date submitted
April 27, 2016
Date released
October 7, 2016
Submitter comment
Kundaje lab found this experiment falls in a relatively large collection of replicates across multiple ZNF TFs that are EXACTLY identical. This is definitely not co-binding TFs since the overlap and concordance is excessively high. It is also impossible to find expected motifs enriched in these peak regions. The peaks seem to be falling in AT repeat regions likely capturing some kind of eGFP ChIPing artifacts. This is confirmed visually in browser sessions.
Tags
Search for all Experiment with internal tag RegulomeDB_2_0Search for all Experiment with internal tag RegulomeDB_1_0Search for all Experiment with internal tag ENCYCLOPEDIAv5

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens ZFP64ENCBS502HSCENCGM466NYUENCAB976SGGENCLB870EUO
21Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens ZFP64ENCBS307QUSENCGM466NYUENCAB976SGGENCLB285SKH