ENCAB976SGG

Homo sapiens

HEK293

characterized to standards

HEK293

Method: immunoblot

compliant

Caption: Western blot analysis of nuclear lysates prepared from HEK293 expressing eGFP-ZNF71-human using anti-GFP antibody ab290. Molecular Weight: 81kDa.
Reviewer comment: While the western blot was passed strictly on the account that the marked signal comprises of >=50% of the total immunoreactive signal in the lane, the presence of multiple bands is not ideal. The lab will try to repeat the characterization on a different eGFP-tagged HEK293 sample that has not already been crosslinked first like this eGFP-ZNF71 one.
Submitted by: Kathrina Onate
Lab: Michael Snyder, Stanford
Grant: U54HG006996
Download: anti-GFP (ab290, Lot GR158277-1) primary image.jpg
Documents: encode:Antibody_Characterization_ENCODE3_August2015.pdf

Method: motif enrichment

compliant

Caption: The motif for target eGFP-CTCF is represented by the attached position weight matrix (PWM) derived from ENCFF955UXT. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.987646461111 Global enrichment Z-score: 11.5201943701 Positional bias Z-score: 14.4108142846 Peak rank bias Z-score: 4.58077718455 Enrichment rank: 1.0
Submitted by: Kathrina Onate
Lab: Michael Snyder, Stanford
Grant: U54HG006996
Download: zzhang:motifpipeline:jobdata:ENCAB976SGG_CTCF_ENCFF955UXT_CTCF-human.png
Documents: encode:TF_Antibody_Characterization_ENCODE3_May2016.pdf