ENCAB976SGG

Antibody against Aequorea victoria eGFP

Homo sapiens
HEK293
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab290
Lot ID
GR158277-1
Host
rabbit
Clonality
polyclonal
External resources

Characterizations

eGFP (Aequorea victoria)
HEK293
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from HEK293 expressing eGFP-ZNF71-human using anti-GFP antibody ab290. Molecular Weight: 81kDa.
Reviewer comment
While the western blot was passed strictly on the account that the marked signal comprises of >=50% of the total immunoreactive signal in the lane, the presence of multiple bands is not ideal. The lab will try to repeat the characterization on a different eGFP-tagged HEK293 sample that has not already been crosslinked first like this eGFP-ZNF71 one.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
eGFP-CTCF (Homo sapiens)
Method: motif enrichment
Attachment from submitter
compliant
Caption
The motif for target eGFP-CTCF is represented by the attached position weight matrix (PWM) derived from ENCFF955UXT. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.987646461111 Global enrichment Z-score: 11.5201943701 Positional bias Z-score: 14.4108142846 Peak rank bias Z-score: 4.58077718455 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford