Functional Characterization Experiment summary for ENCSR763VBG
doi:10.17989/ENCSR763VBG
Summary
- Status
- revoked
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Tiling modality
- peak tiling
- Examined loci
- SUV39H1 (35-45%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-SUV39H1-binC_funccharexp
- Date released
- January 7, 2021
- Submitter comment
- This dataset was revoked because it did not pass the lab's QC assessments.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS204WEL | ENCGM924EDC, ENCGM912CXW | ENCLB645XFE |
| 1 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS204WEL | ENCGM924EDC, ENCGM912CXW | ENCLB779MNL |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS326QPA | ENCGM924EDC, ENCGM912CXW | ENCLB212VFR |
| 3 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS988XMV | ENCGM924EDC, ENCGM912CXW | ENCLB351CFS |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS326QPA | ENCGM924EDC, ENCGM912CXW | ENCLB442BCO |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS204WEL | ENCGM924EDC, ENCGM912CXW | ENCLB013EVL |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS204WEL | ENCGM924EDC, ENCGM912CXW | ENCLB765TJC |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS988XMV | ENCGM924EDC, ENCGM912CXW | ENCLB911PLP |
| 4 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS993UIU | ENCGM924EDC, ENCGM912CXW | ENCLB881LRC |
| 4 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS993UIU | ENCGM924EDC, ENCGM912CXW | ENCLB307ROV |
| 4 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS993UIU | ENCGM924EDC, ENCGM912CXW | ENCLB556NZL |
| 3 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS988XMV | ENCGM924EDC, ENCGM912CXW | ENCLB125JEP |
| 2 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS326QPA | ENCGM924EDC, ENCGM912CXW | ENCLB356HYZ |
| 2 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS326QPA | ENCGM924EDC, ENCGM912CXW | ENCLB311THU |
| 3 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS988XMV | ENCGM924EDC, ENCGM912CXW | ENCLB340USF |
| 4 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS993UIU | ENCGM924EDC, ENCGM912CXW | ENCLB523EUT |