Functional Characterization Experiment summary for ENCSR609RED
doi:10.17989/ENCSR609RED
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PRDX2 (0-10%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-PRDX2-binA_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR006WCB
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS543NBG | ENCGM924EDC, ENCGM912CXW | ENCLB668HHC |
| 1 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS462WHN | ENCGM924EDC, ENCGM912CXW | ENCLB947TTQ |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS543NBG | ENCGM924EDC, ENCGM912CXW | ENCLB770YUQ |
| 2 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS543NBG | ENCGM924EDC, ENCGM912CXW | ENCLB567YET |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS462WHN | ENCGM924EDC, ENCGM912CXW | ENCLB303JSA |
| 1 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS462WHN | ENCGM924EDC, ENCGM912CXW | ENCLB454AID |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS462WHN | ENCGM924EDC, ENCGM912CXW | ENCLB674IVK |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS543NBG | ENCGM924EDC, ENCGM912CXW | ENCLB914RMS |
| 3 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS194VHJ | ENCGM924EDC, ENCGM912CXW | ENCLB394KAP |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS194VHJ | ENCGM924EDC, ENCGM912CXW | ENCLB237SUC |
| 3 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS194VHJ | ENCGM924EDC, ENCGM912CXW | ENCLB933EXU |
| 3 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS194VHJ | ENCGM924EDC, ENCGM912CXW | ENCLB581XKQ |