Functional Characterization Experiment summary for ENCSR550MSE
doi:10.17989/ENCSR550MSE
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PIM2 (90-100%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-PIM2-binF_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR020TLT
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS171DEQ | ENCGM924EDC, ENCGM912CXW | ENCLB040QCT |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS271YOI | ENCGM924EDC, ENCGM912CXW | ENCLB098POW |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS078HRV | ENCGM924EDC, ENCGM912CXW | ENCLB135URI |
| 4 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS154PZI | ENCGM924EDC, ENCGM912CXW | ENCLB080NMX |
| 3 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS078HRV | ENCGM924EDC, ENCGM912CXW | ENCLB178ZWZ |
| 3 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS078HRV | ENCGM924EDC, ENCGM912CXW | ENCLB389LRB |
| 3 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS078HRV | ENCGM924EDC, ENCGM912CXW | ENCLB059SLJ |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS171DEQ | ENCGM924EDC, ENCGM912CXW | ENCLB598LTT |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS271YOI | ENCGM924EDC, ENCGM912CXW | ENCLB345EXD |
| 4 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS154PZI | ENCGM924EDC, ENCGM912CXW | ENCLB036RWD |
| 4 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS154PZI | ENCGM924EDC, ENCGM912CXW | ENCLB332INP |
| 4 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS154PZI | ENCGM924EDC, ENCGM912CXW | ENCLB166ACG |