Functional Characterization Experiment summary for ENCSR545ODU
doi:10.17989/ENCSR545ODU
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- KLF1 (90-100%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-KLF1-binF_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR114BIZ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS587TPX | ENCGM924EDC, ENCGM912CXW | ENCLB166BNE |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS587TPX | ENCGM924EDC, ENCGM912CXW | ENCLB475PKJ |
| 1 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS587TPX | ENCGM924EDC, ENCGM912CXW | ENCLB548BOR |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS074KCV | ENCGM924EDC, ENCGM912CXW | ENCLB623OLL |
| 1 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS587TPX | ENCGM924EDC, ENCGM912CXW | ENCLB377TWT |
| 2 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS074KCV | ENCGM924EDC, ENCGM912CXW | ENCLB767MWL |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS074KCV | ENCGM924EDC, ENCGM912CXW | ENCLB024OFM |
| 2 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS074KCV | ENCGM924EDC, ENCGM912CXW | ENCLB639SDO |