Functional Characterization Experiment summary for ENCSR426GWZ
doi:10.17989/ENCSR426GWZ
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- BAX (55-65%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-BAX-binD_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR473FYG
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS178DWI | ENCGM924EDC, ENCGM912CXW | ENCLB217CGL |
| 1 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS178DWI | ENCGM924EDC, ENCGM912CXW | ENCLB722VTG |
| 2 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS698CQL | ENCGM924EDC, ENCGM912CXW | ENCLB731EMI |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS178DWI | ENCGM924EDC, ENCGM912CXW | ENCLB587GPR |
| 1 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS178DWI | ENCGM924EDC, ENCGM912CXW | ENCLB462TLA |
| 2 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS698CQL | ENCGM924EDC, ENCGM912CXW | ENCLB403UZD |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS698CQL | ENCGM924EDC, ENCGM912CXW | ENCLB206ZHX |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS698CQL | ENCGM924EDC, ENCGM912CXW | ENCLB971YFK |