Functional Characterization Experiment summary for ENCSR218TMF
doi:10.17989/ENCSR218TMF
Summary
- Status
- revoked
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Tiling modality
- peak tiling
- Examined loci
- SUV39H1 (10-20%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-SUV39H1-binB_funccharexp
- Date released
- January 7, 2021
- Submitter comment
- This dataset was revoked because it did not pass the lab's QC assessments.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS445RZX | ENCGM924EDC, ENCGM912CXW | ENCLB135WDM |
| 1 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS445RZX | ENCGM924EDC, ENCGM912CXW | ENCLB340VZF |
| 1 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS445RZX | ENCGM924EDC, ENCGM912CXW | ENCLB182BQR |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS445RZX | ENCGM924EDC, ENCGM912CXW | ENCLB032CSC |
| 3 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS133EJP | ENCGM924EDC, ENCGM912CXW | ENCLB376COV |
| 3 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS133EJP | ENCGM924EDC, ENCGM912CXW | ENCLB492NZA |
| 2 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS877DNC | ENCGM924EDC, ENCGM912CXW | ENCLB738PSI |
| 3 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS133EJP | ENCGM924EDC, ENCGM912CXW | ENCLB537EFG |
| 4 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS000SGE | ENCGM924EDC, ENCGM912CXW | ENCLB304SEU |
| 4 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS000SGE | ENCGM924EDC, ENCGM912CXW | ENCLB746HBE |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS877DNC | ENCGM924EDC, ENCGM912CXW | ENCLB400FBE |
| 4 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS000SGE | ENCGM924EDC, ENCGM912CXW | ENCLB208UTP |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS133EJP | ENCGM924EDC, ENCGM912CXW | ENCLB014LVX |
| 4 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS000SGE | ENCGM924EDC, ENCGM912CXW | ENCLB011WUP |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS877DNC | ENCGM924EDC, ENCGM912CXW | ENCLB167NJQ |
| 2 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS877DNC | ENCGM924EDC, ENCGM912CXW | ENCLB113LDN |