1. Functional Characterization Experiments
  2. Flow-FISH CRISPR screen
  3. Homo sapiens
  4. K562

Functional Characterization Experiment summary for ENCSR080OTF

doi:10.17989/ENCSR080OTF

Summary

Status
revoked
Assay
Flow-FISH CRISPR screen
Biosample summary
Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
Biosample Type
cell line
Tiling modality
peak tiling
Examined loci
SUV39H1 (0-10%)
CRISPR screen readout
PrimeFlow
Replication type
isogenic
Nucleic acid type
DNA
Size range
200-210

Attribution

ENCODE4 project
Lab
Jesse Engreitz, Stanford
Award
UM1HG009436 (Will Greenleaf, Stanford)
Project
ENCODE
Aliases
jesse-engreitz:CRISPRiFlowFISH-K562-SUV39H1-binA_funccharexp
Date released
January 7, 2021
Submitter comment
This dataset was revoked because it did not pass the lab's QC assessments.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
12Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS509OIMENCGM924EDC, ENCGM912CXWENCLB359YXB
44Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS498NWHENCGM924EDC, ENCGM912CXWENCLB730ICU
24Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS516XSKENCGM924EDC, ENCGM912CXWENCLB833TYV
11Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS509OIMENCGM924EDC, ENCGM912CXWENCLB303BSF
21Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS516XSKENCGM924EDC, ENCGM912CXWENCLB439DVQ
23Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS516XSKENCGM924EDC, ENCGM912CXWENCLB475SSQ
34Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS996QSPENCGM924EDC, ENCGM912CXWENCLB789KWD
42Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS498NWHENCGM924EDC, ENCGM912CXWENCLB045QAP
13Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS509OIMENCGM924EDC, ENCGM912CXWENCLB250EPI
14Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS509OIMENCGM924EDC, ENCGM912CXWENCLB864ZQS
41Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS498NWHENCGM924EDC, ENCGM912CXWENCLB291BYO
43Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS498NWHENCGM924EDC, ENCGM912CXWENCLB749ELB
31Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS996QSPENCGM924EDC, ENCGM912CXWENCLB773GUT
33Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS996QSPENCGM924EDC, ENCGM912CXWENCLB805TLD
22Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS516XSKENCGM924EDC, ENCGM912CXWENCLB380OBO
32Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA)ENCBS996QSPENCGM924EDC, ENCGM912CXWENCLB516KVM

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