Functional Characterization Experiment summary for ENCSR048WSZ
doi:10.17989/ENCSR048WSZ
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- PQBP1 (10-20%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-PQBP1-binB_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR245ZZP
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS732PMF | ENCGM924EDC, ENCGM912CXW | ENCLB780FYR |
| 3 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS732PMF | ENCGM924EDC, ENCGM912CXW | ENCLB959QAL |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS732PMF | ENCGM924EDC, ENCGM912CXW | ENCLB804DFO |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS576UYR | ENCGM924EDC, ENCGM912CXW | ENCLB948PKI |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS708TTP | ENCGM924EDC, ENCGM912CXW | ENCLB246WLV |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS708TTP | ENCGM924EDC, ENCGM912CXW | ENCLB310UCA |
| 4 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS503ZUJ | ENCGM924EDC, ENCGM912CXW | ENCLB622LKC |
| 4 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS503ZUJ | ENCGM924EDC, ENCGM912CXW | ENCLB093YDE |
| 4 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS503ZUJ | ENCGM924EDC, ENCGM912CXW | ENCLB320BTW |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS576UYR | ENCGM924EDC, ENCGM912CXW | ENCLB849ELD |
| 3 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS732PMF | ENCGM924EDC, ENCGM912CXW | ENCLB860ZTT |
| 4 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS503ZUJ | ENCGM924EDC, ENCGM912CXW | ENCLB589HQN |