Functional Characterization Experiment summary for ENCSR021HCT
doi:10.17989/ENCSR021HCT
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- HDAC6 (10-20%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-HDAC6-binB_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR541HXR
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS184VHI | ENCGM924EDC, ENCGM912CXW | ENCLB770WNH |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS184VHI | ENCGM924EDC, ENCGM912CXW | ENCLB686KHK |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS092KUC | ENCGM924EDC, ENCGM912CXW | ENCLB362ITN |
| 3 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS092KUC | ENCGM924EDC, ENCGM912CXW | ENCLB630LSZ |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS128CNX | ENCGM924EDC, ENCGM912CXW | ENCLB740UGQ |
| 3 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS092KUC | ENCGM924EDC, ENCGM912CXW | ENCLB474FEE |
| 4 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS912ONR | ENCGM924EDC, ENCGM912CXW | ENCLB575EDX |
| 3 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS092KUC | ENCGM924EDC, ENCGM912CXW | ENCLB545QXC |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS128CNX | ENCGM924EDC, ENCGM912CXW | ENCLB907HRN |
| 4 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS912ONR | ENCGM924EDC, ENCGM912CXW | ENCLB003DCF |
| 4 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS912ONR | ENCGM924EDC, ENCGM912CXW | ENCLB071JDL |
| 4 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS912ONR | ENCGM924EDC, ENCGM912CXW | ENCLB616HIU |