Experiment summary for ENCSR642GBC
doi:10.17989/ENCSR642GBC
Superseded by ENCSR865UKM
Supersedes ENCSR410MIQ
Summary
- Status
- archived
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Target
- RPS3
- Biosample summary
- Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens RPS3
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against RPS3. (RPS3-BGKcLV04-B)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 8.8 [1-1]8.9 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:RPS3-BGKcLV04-B
- Date submitted
- November 20, 2017
- Date released
- December 6, 2017
- Gene Silencing Series
- ENCSR225PTB
- Submitter comment
- As of May 24th, 2018, this experiment has superseded experiment ENCSR410MIQ. As of November 2022, this dataset is superseded by ENCSR865UKM, which includes spike-ins.
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens RPS3 | ENCBS640DOL | ENCGM863LEY | ENCLB072HVI |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens RPS3 | ENCBS515MKD | ENCGM863LEY | ENCLB715ELA |