Experiment summary for ENCSR772DUM
doi:10.17989/ENCSR772DUM
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 genetically modified (deletion) using CRISPR
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control CRISPR gRNA against no target in K562 cells followed by RNA-seq. (NT-BGKcLV04-B)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.1
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT-BGKcLV04-B
- Date submitted
- November 20, 2017
- Date released
- December 9, 2017
- Gene Silencing Series
- ENCSR225PTB , ENCSR506QBE
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR | ENCBS981XHM | ENCGM474NKS | ENCLB818QII |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR | ENCBS906RHA | ENCGM474NKS | ENCLB606CNC |