ENCBS640DOL / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens RPS3
Description
RNA-seq on K562 cells treated with a CRISPR gRNA against RPS3. (RPS3-BGKcLV04-25B)
Product ID
CCL-243
Lot ID
59300853
Culture start date
2016-06-16
Culture harvest date
2016-06-28

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:RPS3-BGKcLV04-25B
Tags
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Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM863LEYdeletionrepressionCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

general protocol
Description: Biosample protocol for RPS3-BGKcLV04-25B
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
immunoblot
Caption excerpt: Western blot following CRISPR against RPS3 in K562 whole cell lysate using…
Attachment from submitter
Caption
Western blot following CRISPR against RPS3 in K562 whole cell lysate using RPS3 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS3. RPS3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn