Experiment summary for ENCSR312HJY
doi:10.17989/ENCSR312HJY
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Target
- CNOT8
- Biosample summary
- Homo sapiens K562 expressing RNAi targeting H. sapiens CNOT8
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with an shRNA knockdown against CNOT8. (K-CNOT8)
- Nucleic acid type
- RNA
- RNA integrity number
- 10 [2-1]9.9 [1-1]
- Depleted in
- rRNA
- Size range
- >200
- Extraction method
- Trizol
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- References
- Aliases
- brenton-graveley:K-CNOT8
- Date submitted
- June 17, 2014
- Date released
- June 30, 2014
- Gene Silencing Series
- ENCSR434OUG
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line expressing RNAi targeting H. sapiens CNOT8 | ENCBS374DHY | ENCGM949ASE | ENCLB321TNS |
| 1 | 1 | Homo sapiens K562 cell line expressing RNAi targeting H. sapiens CNOT8 | ENCBS894CLK | ENCGM949ASE | ENCLB093MJI |