ENCBS374DHY / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line expressing RNAi targeting H. sapiens CNOT8
Description
K562 cells treated with an shRNA knockdown against CNOT8, used in RNA-seq experiments. (K-CNOT8-2)
Product ID
CCL-243
Lot ID
59300853
Culture harvest date
2013-06-10

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:BGK0019B-20, brenton-graveley:K-CNOT8-2
Tags
Visit collection page for ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM949ASEinterferencerepressionRNAitransient transfection

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn
transfection protocol
Description: Transfection protocol for shRNA transfection in K562 cells
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn