Experiment summary for ENCSR898NWE
doi:10.17989/ENCSR898NWE
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in K562 cells followed by RNA-seq
- Nucleic acid type
- RNA
- RNA integrity number
- 9.7 [1-1]9.9 [2-1]
- Depleted in
- rRNA
- Size range
- >200
- Extraction method
- Trizol
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:K-non-target
- Date submitted
- February 6, 2014
- Date released
- June 30, 2014
- Gene Silencing Series
- ENCSR243LLN , ENCSR777JPW , ENCSR434OUG , ENCSR996MPK , ENCSR998CWN , ENCSR917NYB , ENCSR017ZQT
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS322JPK | ENCGM266AWP | ENCLB752PAC |
| 2 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS698ZKR | ENCGM266AWP | ENCLB380KQV |