ENCBS698ZKR / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line expressing RNAi
Description
The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. This K562 growth has been treated with a control shRNA.
Product ID
CCL-243
Lot ID
59300853

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Brenton Graveley
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:K-non-target-2, brenton-graveley:BGK0019B-6
Tags
Search for all Biosample with internal tag ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM266AWPinterferencenon-specific target controlRNAi

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Donor external identifiers
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
References

Documents

growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn
transfection protocol
Description: Transfection protocol for shRNA transfection in K562 cells
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn
general protocol
Description excerpt: Graveley Lab shRNA knockdown followed by RNA-seq Library Preparation and…
Description
Graveley Lab shRNA knockdown followed by RNA-seq Library Preparation and Characterization Document
Submitted by
Julie Wong
Lab
Brenton Graveley, UConn