ENCBS862RAU / primary cell

Summary

Status
released
Term name
activated CD4-positive, alpha-beta T cell
Term ID
CL:0000896
Summary
Homo sapiens male adult (35 years) activated CD4-positive, alpha-beta T primary cell treated with 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 24 hours, anti-CD3 and anti-CD28 coated beads for 14 days

Attribution

ENCODE4 project
Lab
Yijun Ruan, JAX
Award PI
Yijun Ruan, JAX
Submitted by
Bonita Lam
Source
AllCells
Project
ENCODE
Aliases
yijun-ruan:CD4-Naive-(CD45RO-CCR7+)-Day13-Activation_Th0_LP-18

Treatment details

Treatment
10 ng/mL Interleukin-2 (UniProtKB:P60568) for 14 days
Type
protein
Purpose
stimulation
Treatment
anti-CD3 and anti-CD28 coated beads for 24 hours
Type
antibody
Purpose
activation
Treatment
anti-CD3 and anti-CD28 coated beads for 14 days
Type
antibody
Purpose
activation

Donor information

Status
released
Accession
ENCDO374BBL
Aliases
yijun-ruan:donor_LP-18
Donor external identifiers
  • AllCells:10477
Species
Homo sapiens
Life stage
adult
Age
35 years
Sex
male
External resources
Submitter comment
AllCells:10477

Documents

cell isolation protocol
Description excerpt: Protocol describing the purification, activation, and differentiation of…
Description
Protocol describing the purification, activation, and differentiation of various immune cells. This document is an updated version of /documents/f1b60b4e-b5d2-4b8d-af0b-06b767fa07c3/.
Submitted by
Jennifer Jou
Lab
Charles Lee