ENCBS755CSE / primary cell
Summary
- Status
- released
- Term name
- activated CD4-positive, alpha-beta T cell
- Term ID
- CL:0000896
- Summary
- Homo sapiens male adult (35 years) activated CD4-positive, alpha-beta T primary cell treated with 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 24 hours, anti-CD3 and anti-CD28 coated beads for 14 days nuclear fraction
- Subcellular fraction
- nucleus
- Subcellular fraction ID
- GO:0005634
- Starting amount
- 4000000cells
- Separated from biosample
- ENCBS862RAU
Attribution
ENCODE4 project
- Lab
- Charles Lee
- Award PI
- Charles Lee
- Submitted by
- Bonita Lam
- Source
- AllCells
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:CD4-Naive-Th0-Day13-Activation_LP-18_2
Treatment details
- Treatment
- 10 ng/mL Interleukin-2 (UniProtKB:P60568) for 14 days
- Type
- protein
- Purpose
- stimulation
- Treatment
- anti-CD3 and anti-CD28 coated beads for 24 hours
- Type
- antibody
- Purpose
- activation
- Treatment
- anti-CD3 and anti-CD28 coated beads for 14 days
- Type
- antibody
- Purpose
- activation
Donor information
- Status
- released
- Accession
- ENCDO374BBL
- Aliases
- yijun-ruan:donor_LP-18
- Donor external identifiers
- AllCells:10477
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 35 years
- Sex
- male
- External resources
- Submitter comment
- AllCells:10477
Documents
cell isolation protocol
Description excerpt: Protocol describing the purification, activation, and differentiation of…
- Description
- Protocol describing the purification, activation, and differentiation of various immune cells. This document is an updated version of /documents/f1b60b4e-b5d2-4b8d-af0b-06b767fa07c3/.
- Submitted by
- Jennifer Jou
- Lab
- Charles Lee
- Grant
- UM1HG009409